Document Type : Review Article


Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


One of the best and most important foods in the human diet is milk. So, the presence of nutritional compounds and the absence of harmful components is very remarkable. Mycotoxins are an important toxin in food and feed, they are produced by molds. The presence of mycotoxins in food is an emerging issue in the world. Among different types of mycotoxins, aflatoxins are so considerable. Different methods are used to reduce them, but the usage of microbial methods such as Lactic acid bacteria, probiotics, and yeasts is a beneficial strategy. The most studied microorganisms are lactic acid bacteria due to their natural presence in milk and also are known as a GRAS (generally recognized as safe) substances. It is noteworthy to mention that the bacterial cell walls are so important in binding ability. Also, parameters such as pH, temperature of incubation, type of starter, which is used in the product, concentration of microorganisms in milk, and the level of toxin can affect the efficiency of microorganisms. Hence, this review was aimed to investigate the results of studies about the effectiveness of microbial methods on the adsorption and reduction of aflatoxin in milk. Also compares the effect of microorganisms, influencing factors, and mechanisms of these methods.


  1. Iqbal SZ, Jinap S, Pirouz A, Faizal AA. Aflatoxin M1 in milk and dairy products, occurrence and recent challenges: A review. Trends in Food Science & Technology. 2015;46(1):110-9.
  2. Montaseri H, Arjmandtalab S, Dehghanzadeh G, Karami S, Razmjoo M, Sayadi M, et al. Effect of production and storage of probiotic yogurt on aflatoxin M1 residue. Journal of Food Quality and Hazards Control. 2014;1(1):7-14.
  3. Assaf JC, Nahle S, Chokr A, Louka N, Atoui A, El Khoury A. Assorted methods for decontamination of aflatoxin M1 in milk using microbial adsorbents. Toxins. 2019;11(6):304.
  4. Khaneghah AM, Chaves RD, Akbarirad H. Detoxification of aflatoxin M1 (AFM1) in dairy base beverages (acidophilus milk) by using different types of lactic acid bacteria-mini review. Current Nutrition & Food Science. 2017;13:78-81.
  5. International Agency for Research on Cancer. Monograph on the evaluation of carcinogenic risk to humans, world health organization. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. Summary of data reported and evaluation. 2002;82:171-5.
  6. Wu Q, Jezkova A, Yuan Z, Pavlikova L, Dohnal V, Kuca K. Biological degradation of aflatoxins. Drug Metabolism Reviews. 2009;41(1):1-7.
  7. Prandini A, Tansini G, Sigolo S, Filippi L, Laporta M, Piva G. On the occurrence of aflatoxin M1 in milk and dairy products. Food and Chemical Toxicology. 2009;47(5):984-91.
  8. Khiavi NMN, Khiabani MS, Mokarram RR, Kafil HS. Reduction of aflatoxin M1 using mixture of Saccharomyces cerevisiae and Candida albicans cell walls immobilized on silica nanoparticles entrapped in alginate gel. Journal of Environmental Chemical Engineering. 2020;8(1):103635.
  9. Campagnollo FB, Ganev KC, Khaneghah AM, Portela JB, Cruz AG, Granato D, et al. The occurrence and effect of unit operations for dairy products processing on the fate of aflatoxin M1: A review. Food Control. 2016;68:310-29.
  10. European Commission. Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. 2006;364(365–324).
  11. Joint FAO/WHO Expert Committee on Food Additives. Evaluation of certain mycotoxins in food: fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives: World Health Organization; 2002.
  12. Murphy PA, Hendrich S, Landgren C, Bryant CM. Food mycotoxins: an update. Journal of Food Science. 2006;71(5):R51-R65.
  13. Carraro A, De Giacomo A, Giannossi M, Medici L, Muscarella M, Palazzo L, et al. Clay minerals as adsorbents of aflatoxin M1 from contaminated milk and effects on milk quality. Applied Clay Science. 2014;88:92-9.
  14. Tacconi C, Cucina M, Zadra C, Gigliotti G, Pezzolla D. Plant nutrients recovery from aflatoxin B1 contaminated corn through co-composting. Journal of Environmental Chemical Engineering. 2019;7(2):103046.
  15. Bata Á, Lásztity R. Detoxification of mycotoxin-contaminated food and feed by microorganisms. Trends in Food Science & Technology. 1999;10(6-7):223-8.
  16. Colombo M, Castilho NP, Todorov SD, Nero LA. Beneficial properties of lactic acid bacteria naturally present in dairy production. BMC Microbiology. 2018;18(1):219.
  17. Faraki A, Noori N, Gandomi H, Banuree SAH, Rahmani F. Effect of Auricularia auricula aqueous extract on survival of Lactobacillus acidophilus La‐5 and Bifidobacterium bifidum Bb‐12 and on sensorial and functional properties of synbiotic yogurt. Food Science & Nutrition. 2020;8(2):1254-63.
  18. Ismail A, Riaz M, Akhtar S, Ismail T, Ahmad Z, Hashmi MS. Estimated daily intake and health risk of heavy metals by consumption of milk. Food Additives & Contaminants: Part B. 2015;8(4):260-5.
  19. Elsanhoty RM, Salam SA, Ramadan MF, Badr FH. Detoxification of aflatoxin M1 in yoghurt using probiotics and lactic acid bacteria. Food Control. 2014;43:129-34.
  20. Peltonen K, El-Nezami H, Haskard C, Ahokas J, Salminen S. Aflatoxin B1 binding by dairy strains of lactic acid bacteria and bifidobacteria. Journal of Dairy Science. 2001;84(10):2152-6.
  21. Haskard CA, El-Nezami HS, Kankaanpää PE, Salminen S, Ahokas JT. Surface binding of aflatoxin B1 by lactic acid bacteria. Applied and Environmental Microbiology. 2001;67(7):3086-91.
  22. Thyagaraja N, Hosono A. Binding properties of lactic acid bacteria from ‘Idly’towards food-borne mutagens. Food and Chemical Toxicology. 1994;32(9):805-9.
  23. Martínez M, Magnoli A, Pereyra MG, Cavaglieri L. Probiotic bacteria and yeasts adsorb aflatoxin M1 in milk and degrade it to less toxic AFM1-metabolites. Toxicon. 2019;172:1-7.
  24. Gao X, Ma Q, Zhao L, Lei Y, Shan Y, Ji C. Isolation of Bacillus subtilis: screening for aflatoxins B 1, M 1, and G 1 detoxification. European Food Research and Technology. 2011;232(6):957.
  25. Ismail A, Levin RE, Riaz M, Akhtar S, Gong YY, de Oliveira CA. Effect of different microbial concentrations on binding of aflatoxin M1 and stability testing. Food Control. 2017;73:492-6.
  26. Sadeghi A, Ebrahimi M, Sadeghi B. Effect of isolated Lactobacillus acidophilus and Lactobacillus brevis on growth of Aspergillus flavus and reduction of Aflatoxin B1. Journal of Rafsanjan University of Medical Sciences. 2016;15(1):3-16.
  27. Fazeli MR, Hajimohammadali M, Moshkani A, Samadi N, Jamalifar H, Khoshayand MR, et al. Aflatoxin B1 binding capacity of autochthonous strains of lactic acid bacteria. Journal of Food Protection. 2009;72(1):189-92.
  28. Abdelmotilib NM, Hamad GM, Elderea HB, Salem EG, El Sohaimy SA. Aflatoxin M1 reduction in milk by a novel combination of probiotic bacterial and yeast strains. European Journal of Nutrition & Food Safety. 2018:83-99.
  29. Serrano-Niño J, Cavazos-Garduño A, Hernandez-Mendoza A, Applegate B, Ferruzzi M, San Martin-González M, et al. Assessment of probiotic strains ability to reduce the bioaccessibility of aflatoxin M1 in artificially contaminated milk using an in vitro digestive model. Food Control. 2013;31(1):202-7.
  30. Kabak B, Var I. Factors affecting the removal of aflatoxin M1 from food model by Lactobacillus and Bifidobacterium strains. Journal of Environmental Science and Health, Part B. 2008;43(7):617-24.
  31. Line J, Brackett R. Factors affecting aflatoxin B1 removal by Flavobacterium aurantiacum. Journal of Food Protection. 1995;58(1):91-4.
  32. El-Nezami H, Chrevatidis A, Auriola S, Salminen S, Mykkänen H. Removal of common Fusarium toxins in vitro by strains of Lactobacillus and Propionibacterium. Food Additives & Contaminants. 2002;19(7):680-6.
  33. Sarlak Z, Rouhi M, Mohammadi R, Khaksar R, Mortazavian AM, Sohrabvandi S, et al. Probiotic biological strategies to decontaminate aflatoxin M1 in a traditional Iranian fermented milk drink (Doogh). Food Control. 2017;71:152-9.
  34. Karazhiyan H, Mehraban SM, Karazhyan R, Mehrzad A, Haghighi E. Ability of different treatments of Saccharomyces cerevisiae to surface bind aflatoxin M1 in yoghurt. Journal of Agricultural Science and Technology. 2016;18: 1489-98.
  35. Shetty PH, Jespersen L. Saccharomyces cerevisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends in Food Science & Technology. 2006;17(2):48-55.
  36. Devegowda G, Aravind B, Morton M, editors. Saccharomyces cerevisiae and mannanoligosaccharides to counteract aflatoxicosis in broilers. Proceedings of Australian Poultry Science Symposium Sydney; 1996.
  37. Salim A, Zohair A, Hegazy AE-S, Said A. Effect of some strains of probiotic bacteria against toxicity induced by aflatoxins in vivo. The Journal of American Science. 2011;7(1):1-12.
  38. Foroughi M, Sarabi Jamab M, Keramat J, Foroughi M. Immobilization of Saccharomyces cerevisiae on perlite beads for the decontamination of aflatoxin M1 in milk. Journal of Food Science. 2018;83(7):2008-13.
  39. Corassin C, Bovo F, Rosim R, Oliveira C. Efficiency of Saccharomyces cerevisiae and lactic acid bacteria strains to bind aflatoxin M1 in UHT skim milk. Food Control. 2013;31(1):80-3.
  40. Farbo MG, Urgeghe PP, Fiori S, Marceddu S, Jaoua S, Migheli Q. Adsorption of ochratoxin A from grape juice by yeast cells immobilised in calcium alginate beads. International Journal of Food Microbiology. 2016;217:29-34.
  41. Bovo F, Corassin CH, Rosim RE, de Oliveira CA. Efficiency of lactic acid bacteria strains for decontamination of aflatoxin M 1 in phosphate buffer saline solution and in skimmed milk. Food and Bioprocess Technology. 2013;6(8):2230-4.
  42. El-Deeb S, Zaki N, Shoukry Y, Khedr E. Effect of some technological processes on stability and distribution of aflatoxin M1 in milk. Egyptian Journal of Food Science (Egypt). 1992.
  43. El-Nezami H, Kankaanpaa P, Salminen S, Ahokas J. Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin B1. Food and Chemical Toxicology. 1998;36(4):321-6.
  44. El-Nezami H, Kankaanpää P, Salminen S, Ahokas J. Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. Journal of Food Protection. 1998;61(4):466-8.
  45. Pierides M, El-Nezami H, Peltonen K, Salminen S, Ahokas J. Ability of dairy strains of lactic acid bacteria to bind aflatoxin M1 in a food model. Journal of Food Protection. 2000;63(5):645-50.
  46. Hernandez‐Mendoza A, Guzman‐de‐Peña D, Garcia H. Key role of teichoic acids on aflatoxin B1 binding by probiotic bacteria. Journal of Applied Microbiology. 2009;107(2):395-403.
  47. Begley M, Gahan CG, Hill C. The interaction between bacteria and bile. FEMS Microbiology Reviews. 2005;29(4):625-51.
  48. Bron PA, Molenaar D, de Vos WM, Kleerebezem M. DNA micro‐array‐based identification of bile‐responsive genes in Lactobacillus plantarum. Journal of Applied Microbiology. 2006;100(4):728-38.
  49. Whitehead K, Versalovic J, Roos S, Britton RA. Genomic and genetic characterization of the bile stress response of probiotic Lactobacillus reuteri ATCC 55730. Applied and Environmental Microbiology. 2008;74(6):1812-9.
  50. Gunn JS. Mechanisms of bacterial resistance and response to bile. Microbes and Infection. 2000;2(8):907-13.
  51. Leverrier P, Dimova D, Pichereau V, Auffray Y, Boyaval P, Jan G. Susceptibility and adaptive response to bile salts in Propionibacterium freudenreichii: physiological and proteomic analysis. Applied and Environmental Microbiology. 2003;69(7):3809-18.
  52. Dalié D, Deschamps A, Richard-Forget F. Lactic acid bacteria–Potential for control of mould growth and mycotoxins: A review. Food Control. 2010;21(4):370-80.
  53. Maryamma K, Rajan A, Gangadharan B, Ismail P, Valsala K, Manomohan C. Reduction of aflatoxin in milk by fermentation into curd. Journal of Veterinary and Animal Sciences. 1990;21(2):102-7.
  54. Megalla S, Hafez A. Detoxification of aflatoxin B 1 by acidogenous yoghurt. Mycopathologia. 1982;77(2):89-91.
  55. Yousef A, Marth E. Stability and degradation of aflatoxin M1. Mycotoxins in dairy products, edited by HP van Egmond. NEW York: Elsevier; 1989.
  56. Govaris A, Roussi V, Koidis P, Botsoglou N. Distribution and stability of aflatoxin M1 during production and storage of yoghurt. Food Additives & Contaminants. 2002;19(11):1043-50.
  57. Lee Y, El-Nezami H, Haskard C, Gratz S, Puong K, Salminen S, et al. Kinetics of adsorption and desorption of aflatoxin B1 by viable and nonviable bacteria. Journal of Food Protection. 2003;66(3):426-30.