Introduction: In recent decades, there has been a great deal of attention toward the beneficial effects of herbs in prevention and treatment of different diseases. Phytochemicals, flavonoids, phenolic compounds, and antioxidants are most common words in this era. Some of herbs due to their long history of usage are more interesting to be examined and found out their possible medicinal capabilities which in this study antioxidant activity, total phenolic and flavonoids contents of Orthosiphon stamineus, Teucrium polium, and Berberis vulgaris were analyzed.
Materials and Methods: Decoction of the herbs were prepared and analyzed. Total Phenolic Content (TPC) were evaluated with Folin-Ciocalteu's phenol reagent. Colorimetric Aluminum Chloride method was used for flavonoid determination and The antioxidant activities of the extracts were determined on their ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals, based on a modified method of Brand Williams.
Results: Based on the obtained results B. vulgaris showed the highest level of TPC, TFC and antioxidant activity followed by O. stamineus, T. polium. No degradation of analyzed activities were found after mixing all these decoctions.
Conclusion: According to the present study, results of TPC, TFC, and DPPH scavenging activities showed possible beneficial effects of studies herbs and their mixed ones due to their components which might be used in prevention, suppression or treatment of NCDs.